The same sequence in three-letter code is Tyr-Gly-Gly-Phe-Nva-Met-Arg-Phe. In the following example Norlvaline (Nva) is inserted after the fourth Phenylalanine residue. This is useful when an unusual amino-acid, lacking one-letter code, needs to be inserted in otherwise one-letter encoded sequence. Following is the same peptide sequence in three-letter code.įurthermore, peptide calculator allows mixing one-letter and three letter codes. When using three-letter amino acid code, dashes separating one amino-acid from the next are required. With one-letter amino acid code, dashes between adjoining amino acids are optional and can be used for clarity. The shortest way of encoding peptide and protein fragment sequences that peptide calculator understands is through the standard one-letter amino acid codes that are available for usual amino acids, like in the following example of a seven-amino acid peptide. This guide shows what is possible with peptide calculator and gives general tips on how to employ the analytical instrument to your advantage in the fields of biotechnology, molecular biology, and proteomics. Just place a sequence in the edit box, and press the “Calculate” button. Using the peptide properties calculator analytical tool is very easy. Peptide calculator reads a peptide or a protein fragment sequence and displays: average molecular weight with uncertainty, monoisotopic molecular weight, as well as mass divided by charge values for various stages of protonation and deprotonation (which is useful for mas-spec analysis of charged ions). Find mass of amino acid sequences with modifications, isotopic labeling, cyclisation, branching, and more.
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